Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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Doença de Aujeszky
Agarose gel electrophoresis was used to detect PCR products. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. The analytical sensitivity of the test was consistently observed to be 1. PCR experiments were performed on serial ten-fold dilutions of a viral suspension of Aujeszku isolate V with a titer of 10 6.
Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. Support Center Support Center. aujesky
World Organization for Animal Health. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs Also, the BLAST search against nucleotide databases of different herpesvirus and dona nucleotide sequences revealed this region is very specific for PRV genomes.
The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. Published online Sep 1. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.
The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection.
In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. Iowa State University Press; Thus, the optimal concentration of MgCl2 and primers were 1. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I.
The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products. The assay proved to be very sensitive due to as little as 1.
Doens virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8.
This assay was based on the amplification of a highly conserved viral gD gene fragment. Negative controls were run with each test.
The annealing temperature and number of cycles were determined experimentally. This region was highly conserved for all reported genomes as shown by aligning of these sequences. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies. The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. However, this method is time-consuming and false negative results may occur in submissions from latently infected animals The viral agent following a primary replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6.
Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
Non-specific reactions were not observed when a suino herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine. The PCR assay described here provides a rapid, doea sensitive, and cost-effective laboratory diagnosis for pseudorabies infections.
The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome Author information Article notes Copyright and License information Disclaimer. Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction. Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency. Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine.
Can J Com Med. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.